Flow cytometric analysis of Mouse IL-7Rα (CD127) expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 antibody and either Biotin Rat IgG2a, κ Isotype Control (Left panel) or SDT Biotin Rat Anti-Mouse IL-7Rα (CD127) Antibody (Right panel) at 5 μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse IL-7Rα (CD127) Antibody (S-3263)
Biotin Rat Anti-Mouse IL-7Rα (CD127) Antibody (S-3263)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-7Rα (CD127) |
| Synonyms | Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CD127; Il7r |
| Location | Membrane |
| Accession | P16872 |
| Clone Number | S-3263 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
Interleukin-7 receptor α chain (IL-7Rα, CD127) is a 75 kDa type-I transmembrane glycoprotein that forms the cytokine-binding subunit of both the IL-7 receptor (IL-7R) and the thymic stromal lymphopoietin receptor (TSLPR); upon binding IL-7 it heterodimerizes with the common gamma chain (γc, CD132) to initiate JAK1/JAK3-mediated phosphorylation of STAT5, thereby driving survival, proliferation and differentiation of T, B and innate lymphoid cells, while TSLP engagement pairs IL-7Rα with the TSLPR chain to modulate type-2 immunity.
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