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Biotin Mouse Anti-Human Perforin Antibody (S-3064)

Biotin Mouse Anti-Human Perforin Antibody (S-3064)

Catalog Number: S0B8705 Application: ICFCM Reactivity: Human Conjugation: Biotin Brand: Starter
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$120 USD
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Product Details

Product Specification


Host Mouse
Antigen Perforin
Synonyms Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein (PFP); PFP; PRF1
Location Secreted, Cell membrane
Accession P14222
Clone Number S-3064
Antibody Type Mouse mAb
Isotype IgG2b,k
Application ICFCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Biotin
Physical Appearance Liquid
Storage Buffer

PBS pH7.4, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Hu

Background

Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions as a pore-forming protein, inserting itself into the membranes of target cells, such as virus-infected or cancerous cells. Once integrated, perforin creates pores that disrupt the cell membrane's integrity, leading to osmotic lysis and ultimately killing the target cell. This mechanism is essential for the immune system's ability to eliminate harmful cells and maintain homeostasis.

Picture

FC

Flow cytometric analysis of Perforin expression in PBMC (Human peripheral blood mononuclear cells). Human PBMC were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with Alexa Fluor® 647 Mouse Anti-Human CD4 Antibody and either Biotin Mouse IgG2b, κ Isotype Control or SDT Biotin Mouse Anti-Human Perforin Antibody at 5μl/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.