Flow cytometric analysis of Human Granzyme A expression in Human PBMC. Human PBMC were fixed with 4% PFA and permeabilized with 0.1% Tween. The cells were then stained with Pacific Blue Mouse Anti-Human CD8 Antibody and either Biotin Mouse IgG1, κ Isotype Control (Left panel) or Biotin Mouse Anti-Human Granzyme A Antibody (Right panel) at 5 μl/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 777 (S0D0026), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human Granzyme A Antibody (S-3175)
Biotin Mouse Anti-Human Granzyme A Antibody (S-3175)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Granzyme A |
| Synonyms | CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor (H factor; HF); CTLA3; HFSP; GZMA |
| Location | Secreted |
| Accession | P12544 |
| Clone Number | S-3175 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Hu |
Background
Granzyme A (GZMA) is a 28 kDa serine protease stored in the cytotoxic granules of CD8⁺ T cells and NK cells; upon immunological synapse formation, it enters target cells together with perforin and cleaves after Lys or Arg residues to hydrolyze SET complex proteins (including the SET, APE1, and HMGB2 nucleases), thereby disrupting DNA repair and initiating a caspase-independent, oxidative DNA-damage cell death pathway that manifests as single-stranded DNA nicks, mitochondrial dysfunction, and plasma membrane perforation, while extracellularly it can process extracellular matrix components and cytokine precursors to modulate inflammation, vascular integrity, and antiviral immunity.
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