Flow cytometric analysis of CD66a/c/e expression on human peripheral blood leukocytes. Human peripheral blood leukocytes were stained with APC Mouse IgG2b, k Isotype Control (left panel) or SDT APC Mouse Anti-Human CD66a/c/e Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
APC Mouse Anti-Human CD66a/c/e Antibody (S-3923)
APC Mouse Anti-Human CD66a/c/e Antibody (S-3923)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD66a/c/e |
| Location | Secreted, Cell membrane |
| Accession | P13688、P40199、P06731 |
| Clone Number | S-3923 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood leukocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD66a/c/e are closely related glycoproteins of the carcinoembryonic-antigen family that share an N-terminal IgV-like domain followed by up to three IgC2-like domains, differ mainly in membrane anchorage (CD66a is transmembrane whereas CD66c and CD66e are GPI-linked), and collectively mediate calcium-independent homophilic and heterophilic adhesion, pathogen recognition (e.g., gonococcal Opa proteins bind peptide determinants in their N-domains), neutrophil activation, and modulation of innate and adaptive immunity, apoptosis, angiogenesis, and tumor behavior.
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