Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling APC-Cy7 Mouse IgG2a, κ Isotype Control Antibody at 1 μg/test treated with True-Stain Monocyte Blocker™ (red line histogram) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC-Cy7 Mouse IgG2a, κ Isotype Control (S-844-17)
APC-Cy7 Mouse IgG2a, κ Isotype Control (S-844-17)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2a,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | APC-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SDT APC-Cy7 Mouse IgG2a, κ Isotype Control Antibody at 1 μg/test treated with True-Stain Monocyte Blocker™ (red line histogram) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) labeling APC-Cy7 Mouse IgG2a, κ Isotype Control Antibody at 1 μg/test treated with True-Stain Monocyte Blocker™ (red line histogram) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
