Flow cytometric analysis of Mouse FR4 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD4 Antibody and either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Left panel) or Alexa Fluor® 647 Rat Anti-Mouse FR4 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse FR4 Antibody (S-R705)
Alexa Fluor® 647 Rat Anti-Mouse FR4 Antibody (S-R705)
Price:
Regular price
$210 USD
Regular price
Sale price
$210 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | FR4 |
| Synonyms | Sperm-egg fusion protein Juno; Folate receptor 4; Folate receptor delta (FR-delta); Folate-binding protein 3; IZUMO1 receptor protein JUNO; Folbp3; Folr4; Juno; Izumo1r |
| Location | Cell membrane |
| Accession | Q9EQF4 |
| Clone Number | S-R705 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
Folate receptor 4 (FR4), also known as folate receptor delta or IZUMO1R, is a protein encoded by the FOLR4 gene in humans. It is a subtype of the receptor for the vitamin folic acid and is primarily expressed on CD4 T lymphocytes, with lower expression on CD8 T cells. FR4 is highly expressed in regulatory T cells (Tregs) and is also found in tumor-associated macrophages (TAMs) and T follicular helper cells (TFH cells). This protein plays a role in immune regulation, as its high expression can distinguish Tregs from other T cells. Additionally, FR4 is involved in the high-affinity binding and transport of folate and folate analogs across the cell membrane.
Picture
Picture
FC
