Flow cytometric analysis of Rat CD1d expression on Lewis Rat thymocytes. Lewis Rat thymocytes were stained with Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Rat CD1d Antibody (Red line histogram) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Rat CD1d Antibody (S-R701)
Alexa Fluor® 488 Mouse Anti-Rat CD1d Antibody (S-R701)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD1d |
| Synonyms | Antigen-presenting glycoprotein CD1d; Cd1d1; Cd1d |
| Location | Cell membrane, Endoplasmic reticulum |
| Accession | Q63493 |
| Clone Number | S-R701 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | Lewis Rat thymocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Rt |
Background
CD1d is a non-polymorphic, MHC class I-like molecule encoded by the CD1D gene, which is a member of the CD1 family of glycoproteins expressed on the surface of various human antigen-presenting cells. It presents lipid antigens, including phospholipids and glycosphingolipids, to a subset of T cells known as invariant natural killer T (iNKT) cells. When activated by CD1d-presented antigens, iNKT cells rapidly produce Th1 and Th2 cytokines, such as interferon-gamma and interleukin-4. CD1d is also involved in the selection and regulation of iNKT cells. Some known ligands for CD1d include α-galactosylceramide (α-GalCer), a compound derived from a marine sponge, and various microbial and self-antigens.
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