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Human IL-15 Serum&Plasma OneStep OptiQuant Plus ELISA Kit

Human IL-15 Serum&Plasma OneStep OptiQuant Plus ELISA Kit

Catalog Number: S0C3014-HL2 Reactivity: Human Conjugation: Brand: Starter
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Regular price $500 USD
Regular price Sale price $500 USD
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Product Details

Product Specification


Antigen IL-15
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

Kit


Precision Intra-assay: 2.0%;
Inter-assay: 2.4%
Sample type Serum; Plasma
Assay type Sandwich (quantitative)
Sensitivity 1.29 pg/mL
Range 7.81 pg/mL – 500 pg/mL
Recovery Serum: 106%
Citrate plasma: 88%
Assay time 60 minutes
Species reactivity Hu

Background

Interleukin-15 (IL-15) is a pleiotropic cytokine critical for the development, survival, and activation of innate and adaptive immune cells. Structurally, IL-15 is a four-α-helix bundle cytokine belonging to the common γ-chain (γc) family. It exists in both soluble and membrane-bound forms, stabilized by disulfide bonds between conserved cysteine residues. The functional IL-15 protein typically forms a heterotrimeric complex with the IL-15 receptor α-chain (IL-15Rα) and the shared β/γc chains, enabling high-affinity signaling. Post-translational modifications, including glycosylation at specific asparagine residues, enhance its stability and modulate receptor binding dynamics. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IL-15 quantification in time-sensitive or resource-limited settings.

Picture

ELISA

Standard curve
Example of Human IL-15 standard curve in Assay Diluent HA2.

To assess the linearity of the assay, two samples were spiked with high concentrations of Human IL-15 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Leading Competitor comparison

Protocol Diagram