At low concentrations of IL-18BP, the presence of IL-1F7b increased the ability of IL-18BP to neutralize IL-18-induced IFN-γ.
IL-37b/IL-1F7b His Tag Protein, Human
IL-37b/IL-1F7b His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL37,FIL1Z, IL1F7, IL1H4, IL1RP1 |
| Accession | AAH20637.1 |
| Amino Acid Sequence | Val46-Asp218 with His Tag at the C-Terminus |
| Expression System | E. coli |
| Molecular Weight | 15-25kDa（Reducing） |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4,1mM DTT |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation |
| Stability & Storage |
· 12 months from date of receipt,lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃under sterile conditions after reconstitution. · 1 week, 2 to 8℃under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Nold MF, Nold-Petry CA, Zepp JA, Palmer BE, Bufler P, Dinarello CA. IL-37 is a fundamental inhibitor of innate immunity. Nat Immunol. 2010 Nov;11(11):1014-22. 2.Sharma S, Kulk N, Nold MF, Gräf R, Kim SH, Reinhardt D, Dinarello CA, Bufler P. The IL-1 family member 7b translocates to the nucleus and down-regulates proinflammatory cytokines. J Immunol. 2008 Apr 15;180(8):5477-82. 3.Zhang ZZ, Zhang Y, He T, Sweeney CL, Baris S, Karakoc-Aydiner E, Yao Y, Ertem D, Matthews HF, Gonzaga-Jauregui C, Malech HL, Su HC, Ozen A, Smith KGC, Lenardo MJ. Homozygous IL37 mutation associated with infantile inflammatory bowel disease. Proc Natl Acad Sci U S A. 2021 Mar 9;118(10):e2009217118. |
Background
IL-37b (Interleukin-37 isoform b, also known as IL-1F7b, is an anti-inflammatory cytokine in the IL-1 family. It contains a β-trefoil fold composed of 12 β-strands and is the largest and most extensively studied isoform of IL-37. Through the IL-18Rα/IL-1R8 receptor complex and a SMAD3-dependent pathway, it inhibits the production of pro-inflammatory cytokines such as IL-1α and IL-6, and downregulates signaling pathways including NF-κB and PI3K-Akt. Clinical studies have found that IL-37b is abnormally expressed in inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease, and allergic asthma; mouse models show that it has protective effects and is a potential therapeutic target for inflammatory diseases.
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Bioactivity
Recombinant human IL-37b inhibits LPS-induced mouse IL-6 production by RAW 264.7 cells
in a dose-dependent manner. The ED50 for this effect is 0.16-1.00 μg/mL.
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
The purity of IL-37b/IL-1F7b His Tag Protein, Human is more than 90% determined by SEC-HPLC
ELISA
Immobilized IL-37b/IL-1F7b His Tag Protein, Human (Cat. No.UA016197) at 2.0μg/mL (100μL/well) can bind Biotinylated IL-18Rα/IL-1R5 His Tag Protein, Human (Cat. No. UA010914) with EC50 of 0.88-1.22 μg/mL.
