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NRG1 Beta 1 His Tag Protein, Human

NRG1 Beta 1 His Tag Protein, Human

Catalog Number: UA016175 Brand: UA BIOSCIENCE
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$185 USD
$185 USD
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Product Details

Product Specification


Species Human
Synonyms NRG1, ARIA, GGF, HGL, HRGA, NDF, SMDF
Accession Q02297-6
Amino Acid Sequence

Ser 177 - Glu 241 with His Tag at the N-Terminus
Expression System HEK293
Molecular Weight 10-15kDa (Reducing)
Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1. Mei L, Xiong WC. Neuregulin 1 in neural development, synaptic plasticity and schizophrenia. Nature Reviews Neuroscience, 2008, 9(6): 437-452
2. Britsch S. The neuregulin-I/ErbB signaling system in development and disease. Advances in Anatomy, Embryology and Cell Biology, 2007, 190: 1-65.
3. Kucharczyk T, et al. NRG1 Gene Fusions—What Promise Remains Behind These Rare Genetic Alterations? A Comprehensive Review of Biology, Diagnostic Approaches, and Clinical Implications. Cancers, 2024, 16(15): 2766-2780.

Background

NRG1β1 (also known as HRG1-β1, heregulin beta-1) is a soluble isoform of Neuregulin-1 (NRG1) generated by alternative splicing. The NRG1 gene is located on human chromosome 8p12 and belongs to the NRG gene family (NRG1, NRG2, NRG3, NRG4).

NRG1β1 contains an immunoglobulin (Ig)-like domain and an epidermal growth factor (EGF)-like domain. The EGF-like domain, encoded by exons 6 and 7, is essential for its biological activity and receptor binding. Extensive alternative splicing of the NRG1 gene generates α and β variants at the C-terminal region of the EGF-like domain, with isoforms containing the β-variant EGF-like domain exhibiting significantly higher binding affinity for ErbB3 or ErbB4 homodimers than α-variant isoforms.

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

ELISA

Immobilized Human NRG1 Beta 1, His Tag (Cat. No. UA016175) at 2μg/ml (100μl/well) on the plate can bind ErbB3/Her3 Fc Chimera Protein, Human (Cat. No. UA016071) with the EC50 of 4-7.1ng/ml.