1μg (R: reducing conditions, N: non-reducing
conditions).
Biotinylated ACE2 Fc&Avi Tag Protein, Human
Biotinylated ACE2 Fc&Avi Tag Protein, Human
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$560 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | ACE-2, ACEH |
| Accession | Q9BYF1-1 |
| Amino Acid Sequence | Gln18-Ser740, with C-terminal Human IgG1 Fc&Avi Tag |
| Expression System | HEK293 |
| Molecular Weight | 120-130kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Biotin |
| Tag | Human Fc Tag, Avi Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. RENHONG YAN, YUANYUAN ZHANG, YANING LI, LU XIA, YINGYING GUO, AND QIANG ZHOU: Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2, SCIENCE, 4 Mar 2020, Vol 367, Issue 6485, pp. 1444-1448. |
Background
The angiotensin-converting enzyme 2 (ACE2) is the receptor for the three coronaviruses HCoV-NL63, SARS-CoV, and SARS-CoV-2. ACE2 is involved in the regulation of the renin-angiotensin system and blood pressure. ACE2 is also involved in the regulation of several signaling pathways, including integrin signaling. ACE2 expression is regulated transcriptionally and post-transcriptionally. The expression of the gene is regulated by two promoters, with usage varying among tissues. ACE2 expression is greatest in the small intestine, kidney, and heart and detectable in a variety of tissues and cell types. ACE2 receptors are ubiquitous and widely expressed in the heart, vessels, gut, lung (particularly in type 2 pneumocytes and macrophages), kidney, testis and brain. ACE2 is mostly bound to cell membranes and only scarcely present in the circulation in a soluble form. An important salutary function of membrane-bound and soluble ACE2 is the degradation of angiotensin II to angiotensi. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in SARS pathogenesis.
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SDS-PAGE
ELISA
Immobilized SARS-CoV-2 Omicron RBD (BA.2.75), His tag(Cat. No. S0A2028) at 2μg/mL can bind Biotinylated ACE2 Fc&Avi Tag Protein, Human(Cat. No. UA010669) with EC50 of 3.3-5.4 ng/mL.
SPR
Biotinylated ACE2 Fc&Avi Tag Protein, Human (Cat. No. UA010669) captured on Biotin CAP - Series S sensor Chip can bind Spike His Tag Protein, SARS-CoV-2(R682S,R685S,K986P&V987P) (Cat. No. UA030071) with an affinity constant of 0.13μM as determined in a SPR assay.
