Trypan Blue Staining Cell Viability Assay Kit

FYI:
1. Collect cells:
For adherent cells, the lower cells were first digested with trypsin and/or EDTA. For suspended cells, the cells can be collected directly. The collected cells were centrifuged at 1000-2000 g for 1 minute, the supernatant was discarded, and the cells were resuspended with 1 mL or appropriate cell resuspension depending on the amount of cells.  
2. Trypan blue staining:
Draw 100 microliters of resuspended cells into a plastic centrifuge tube, add 100 microliters of trypan blue staining solution (2X), gently mix and stain for 3 minutes (3 minutes of staining is enough, and the staining time can be longer, but it should not exceed 10 minutes).
Note: For cells resuspended in cell culture medium, an equal volume of trypan blue staining solution (2X) can also be directly added for staining.
3. Counting:
A small amount of stained cells was aspirated and counted on a blood count plate. Usually, if quantification is to be performed more accurately, at least 500 cells per cell sample, and the blue cells and the total number of cells are counted. The cell viability rate was calculated as follows:
Cell viability = (total number of cells-number of blue cells)/total number of cells × 100%

        Trypan blue stained cell viability test kit (Trypan Blue Staining Cell Viability Assay Kit ), is developed by using normal healthy cells that can reject trypan blue, while cells that have lost cell membrane integrity can be stained with trypan blue. Trypan blue staining detects the integrity of the cell membrane, It is generally considered that the cell membrane has lost its integrity, that is, the cell is considered dead.
        After trypan blue staining, the cell survival rate can be quantified more accurately by counting directly under the microscope or counting after taking photos under the microscope. After trypan blue staining HeLa Please refer to Figure for cells 1 。
        Trypan blue staining only requires 3-5 It can be done in minutes, and it is very simple to operate.
        This kit is sufficient to detect 100 Cell samples.
Product composition:

1. A small amount of granular precipitate may appear when this product is stored for a long time. The precipitate can be fully dissolved in a 37 °C water bath for about 10 minutes. After the precipitate is completely dissolved, it can be used normally.
2. Trypan blue is toxic to human body. Please be particularly careful when operating, and pay attention to effective protection to avoid direct contact with human body or inhalation.
3. For your safety and health, please wear a laboratory coat and disposable gloves and masks.