PCR Mycoplasma Test Kit

Catalog Number: abs9588 Brand: Absin

Price:

$35 USD

Size: 50T

Quantity:

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Product Details

Product Specification

Usage

1 Sample preparation: Take an appropriate amount of cell culture supernatant to be tested into the clean PCR In the tube, use PCR Instrument 95℃ Heat treatment 5 min After as a template. Serum samples available Mycoplasma Free Water After dilution, take an appropriate amount of sample into a clean PCR In the tube, use PCR Instrument 95℃ Heat treatment 5 min After as a template;
2 、 PCR System Preparation: Each experiment needs to set up a negative control (will 1 μL The sample to be tested is replaced with the same amount Mycoplasma Free Water ) vs. positive control (in 1 μL Add to the sample to be tested 0.5 μL Positive Control Later together as Template ), wear disposable masks and gloves during the experiment, and operate carefully to prevent improper operation from introducing exogenous mycoplasma contamination;

Components	Volume
Template	1ul
Mycoplasma Primer Mix	2ul
Mycoplasma PCR Mix （ 2× ）	10ul
Mycoplasma Free Water	To 20ul

3 、 PCR Program settings:

Step	Temperature	Time	Cycles
Initial Denaturation	98℃	2 min	1
Denaturation	98℃	20 S	30
Annealing	56℃	25 S
Extension	72℃	10 S
Final extension	72℃	5 min	1
Hold	4-16℃	Forever

4 Gel electrophoresis: Take 10 μL PCR Product, using 1% Sepharose gel for electrophoresis detection;
5 Results analysis: In each experiment, the mycoplasma contamination and the size of positive band were confirmed by comparing the test results with the negative control and positive control 500 bp Left and right. If there is a band in the negative control test result, it is likely that PCR There is contamination in the system, so it is recommended to re-test to confirm the results. If necessary, also PCR The products were routinely sequenced to determine specific mycoplasma species.

Description

Mycoplasma contamination will cause adverse effects on all aspects of cells, which has become a highly valued problem in cell culture. Therefore, it is necessary to carry out regular detection of mycoplasma contamination. The mycoplasma physical examination test kit is made of PCR Methods, the contamination of mycoplasma in various cultured cell biomaterials (such as cell culture, experimental animal secretions, animal serum, etc.) was specifically detected.
The mixed primers used in the kit are for Mycoplasma 16s rRNA Specific primers designed for conserved regions of the sequence can be directly used as cell culture fluid PCR Template, specifically amplifying mycoplasma DNA , with matching Mycoplasma PCR Mix （ 2× ) can be completed within one hour. This kit has high detection sensitivity and can detect as low as 20 Copies of mycoplasma.
Product Components:

Components	Name	Specifications
A	Mycoplasma PCR Mix （ 2× ）	1ml
B	Mycoplasma Primer Mix	200ul
C	Positive Control	50ul
D	Mycoplasma Free Water	1ml

General Notes

1. This kit can detect at least 11 mycoplasma contamination such as M.orale, M.arginini, M.bovis, M.fermentans, M.gallisepticum, M.hominis, M.pirum, Ureaplasma spp, M.hyorhinis, M.pneumoniae, A.laidlawii, etc.
2. All reagents should be thoroughly thawed and mixed on ice before use, and stored at-20 ℃ after use.
3. Negative control and positive control must be set in each experiment. It is recommended for the experimental group to set a sample template gradient.
4. Masks must be worn during operation, and PCR operating standards must be strictly followed to prevent the introduction of external pollution from affecting the experimental results.
5. In order to ensure the reliability and stability of cell experiments, it is recommended to conduct regular mycoplasma contamination detection.
6. For your safety and health, please wear a lab coat and disposable gloves.

Storage Temp.

Stored at-20 ℃, shelf life is 12 months.

Country/region

PCR Mycoplasma Test Kit