WB result of V5 Tag Mouse mAb (S-610-66)
Lysate: V5 tagged fusion protein 20 ng
Primary antibody:
Lane 1: V5 Tag Mouse mAb at 1/5000 dilution
Lane 2: V5 Tag Mouse mAb at 1/10000 dilution
Lane 3: V5 Tag Mouse mAb at 1/50000 dilution
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
V5 Tag Mouse mAb (S-610-66)
V5 Tag Mouse mAb (S-610-66)
Price:
Regular price
$70.00 USD
Regular price
Sale price
$70.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Mouse |
| Synonyms | V5 |
| Immunogen | Synthetic Peptide |
| Clone Number | S-610-66 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, ICC |
| Reactivity | Species Independent |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | |
| ICC | 1:500 |
Background
V5-tag is a short peptide tag for detection and purification of proteins. The V5-tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in Histone H3-Flag-V5-transfected 293T cells. Anti-V5 Tag antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
Negative control: ICC shows negative staining in vector-transfected 293T cells. Anti-V5 Tag antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
