Flow cytometric analysis of Jurkat cells labelling Stathmin antibody at 1/250 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody.
Stathmin Recombinant Rabbit mAb (SDT-R023)
Stathmin Recombinant Rabbit mAb (SDT-R023)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Stathmin |
| Synonyms | STMN1, C1orf215, LAP18, OP18, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18 (Op18), Phosphoprotein p19 (pp19), Prosolin |
| Immunogen | N/A |
| Location | Cytoplasm |
| Accession | P16949 |
| Clone Number | SDT-R023 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P, FC |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | null |
| FC | 1:250 | null |
Background
Stathmin, also known as metablastin and oncoprotein 18 is a protein that in humans is encoded by the STMN1 gene. Stathmin is a highly conserved 17 kDa protein that is crucial for the regulation of the cell cytoskeleton. Changes in the cytoskeleton are important because the cytoskeleton is a scaffold required for many cellular processes, such as cytoplasmic organization, cell division and cell motility. More specifically, stathmin is crucial in regulating the cell cycle.
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Validation Data
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-Stathmin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex.
Anti-Stathmin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer.
Anti-Stathmin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain.
Anti-Stathmin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain.
Anti-Stathmin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
