WB result of PMEL/Melanoma gp100 Rabbit mAb Primary antibody: PMEL/Melanoma gp100 Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: SK-MEL-28 whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 70 kDa Observed MW: 100 kDa
PMEL/Melanoma gp100 Recombinant Rabbit mAb (SDT-434-70)
PMEL/Melanoma gp100 Recombinant Rabbit mAb (SDT-434-70)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PMEL/Melanoma gp100 |
| Synonyms | ME20-M, ME20M, Pmel 17, P1, P100, Premelanosome protein, Silver locus protein homolog, Melanosome, HMB45 |
| Immunogen | Synthetic Peptide |
| Location | Melanosome |
| Accession | P40967 |
| Clone Number | SDT-434-70 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICC | 1:250 | |
| IHC-P | 1:1000 |
Background
PMEL encodes a key component of the melanosome, the organelle essential for melanin synthesis, storage and transport. Melanoma gp100 is a protein highly expressed in melanoma tissue.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human melanoma. Anti-PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human transitional cell carcinoma. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human prostatic carcinoma. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in SK-MEL-28 cells. Anti-PMEL/Melanoma gp100 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control:ICC shows negative staining in HeLa cells. Anti-PMEL/Melanoma gp100 antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
