p53 Recombinant Rabbit mAb (SDT-100-72)

Catalog Number: S0B2077 Application: WB,IHC-P,ICC,FCM Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$100.00 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	p53
Synonyms	Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	P04637
Clone Number	SDT-100-72
Antibody Type	Rabbit mAb
Application	WB, IHC-P, ICC, ICFCM
Reactivity	Hu
Predicted Reactivity	TrSw
Purification	Protein A
Concentration	0.25 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
ICC	1:500
ICFCM	1:250
IHC-P	1:1000
WB	1:2500

Background

p53, also known as Tumor protein P53, cellular tumor antigen p53 (UniProt name), or transformation-related protein 53 (TRP53) is a regulatory protein that is often mutated in human cancers. The p53 proteins (originally thought to be, and often spoken of as, a single protein) are crucial in vertebrates, where they prevent cancer formation. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene.

Picture

Validation Data

Flow cytometric analysis of HT-29 cells labelling p53 antibody at 1/250 dilution (0.1ug)/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

Western Blot

WB result of p53 Rabbit mAb

Primary antibody: p53 Rabbit mAb at 1/2500 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: T47D whole cell lysate 20 µg

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 53 kDa
Observed MW: 53 kDa
Exposure time: 10s

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon cancer.

Anti-p53 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer.

Anti-p53 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer.

Anti-p53 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer.

Anti-p53 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HT-29 cells. Anti-p53 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).