WB result of NSE Rabbit mAb
Primary antibody : NSE Rabbit mAb at 1/500 dilution
Lane 1 : Hela whole cell lysate 20 µg
Lane 2 : SK-BR-3 whole cell lysate 20 µg
Negative control: Hela whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
(This blot was developed with high sensitivity substrate)
NSE Recombinant Rabbit mAb (SDT-004-100)
NSE Recombinant Rabbit mAb (SDT-004-100)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NSE |
| Synonyms | ENO2, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, neural enolase |
| Immunogen | Recombinant Protein |
| Accession | P09104 |
| Clone Number | SDT-004-100 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Research Area | Neuroscience |
| Concentration | 0.25mg/ml |
| Molecular Weight | 47kDa |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | null |
| IHC-P | 1:1000 | null |
Background
Neuron specific enolase (NSE) is a phosphopyruvate hydratase and is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. NSE as a serum biomarker has been widely used in diagnosis of various malignant tumors in clinical, and it has been found to be associated with melanoma, seminoma, renal cell carcinoma, Merkel cell tumor, carcinoid tumors, dysgerminomas, and immature teratomas, especially small cell lung cancer (SCLC).
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human pancreas islet. Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human lung adenocarcinoma(negative tissue). Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
