Schematic diagram of Mouse NK Cell Panel Kit (CD45/CD3/CD49b)
Mouse NK Cell Panel Kit (CD45/CD3/CD49b)
Mouse NK Cell Panel Kit (CD45/CD3/CD49b)
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$167 USD
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Ms |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This three‑color panel is designed for detecting NK cells and NKT cells in mouse tissue cells. CD45 serves as a pan-leukocyte marker, while CD3 defines total T cells. CD49b is incorporated as a canonical marker widely used to detect natural killer (NK) cells. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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Picture
FC
Mouse hepatocytes were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)
Mouse pulmonary cells were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)
Mouse cutaneous cells were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)
Mouse splenocytes were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)
Mouse intestinal cells were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)
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