Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with anti-Mouse CD22 antibody at 1/200 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti- Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Mouse Anti-Mouse CD22 Antibody (Cy34.1)
Mouse Anti-Mouse CD22 Antibody (Cy34.1)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD22 |
| Synonyms | B-cell receptor CD22; B-lymphocyte cell adhesion molecule (BL-CAM); Sialic acid-binding Ig-like lectin 2 (Siglec-2); T-cell surface antigen Leu-14; Lyb-8; Siglec2; Cd22 |
| Location | Cell membrane |
| Accession | P35329 |
| Clone Number | Cy34.1 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B0617 |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:200 | Ms |
Background
CD22, also known as cluster of differentiation 22, is a 140 kDa type I transmembrane glycoprotein and a member of the Siglec (sialic acid-binding immunoglobulin-like lectin) family. It is predominantly expressed on the surface of mature B cells and to a lesser extent on some immature B cells. The extracellular domain of CD22 consists of seven immunoglobulin-like domains, while its cytoplasmic tail contains conserved tyrosine motifs, including immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which play a crucial role in inhibiting B cell receptor (BCR) signaling. CD22 functions as a negative regulator of B cell activation, helping to prevent overactivation of the immune system and the development of autoimmune diseases. Additionally, it is involved in B cell trafficking and maintaining peripheral B cell tolerance. CD22's interaction with sialic acid-containing molecules on various cell types allows it to modulate B cell responses and maintain immune homeostasis.
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