Product Specification
| Host |
Mouse |
| Antigen |
CD28 |
| Synonyms |
T-cell-specific surface glycoprotein CD28, TP44 |
| Immunogen |
Recombinant Protein |
| Location |
Cell membrane |
| Accession |
P10747 |
| Clone Number |
S-714-76 |
| Antibody Type |
Mouse mAb |
| Isotype |
IgG1,k |
| Application |
FCM |
| Reactivity |
Hu |
| Purification |
Protein G |
| Concentration |
0.2mg/ml |
| Conjugation |
Alexa Fluor® 647 |
| Physical Appearance |
Liquid |
| Storage Buffer |
PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage |
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application |
dilution |
species |
| FCM |
5 μl per million cells in 100μl volume |
|
Background
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.
