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Alexa Fluor® 647 Mouse Anti-Human CD107b/LAMP2 Antibody (H4B4)

Alexa Fluor® 647 Mouse Anti-Human CD107b/LAMP2 Antibody (H4B4)

Catalog Number: S0B80755 Brand: Starter
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$145 USD
$145 USD
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Product Details

Product Specification


Host Mouse
Antigen CD107b/LAMP2
Synonyms Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LGP-96
Location Cell membrane
Accession P13473
Clone Number H4B4
Antibody Type Mouse mAb
Isotype IgG1,k
Isotype Control S0B5964
Positive Sample Human PBMC
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 647
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.09% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
ICFCM 5μl per million cells in 100μl volume Hu

Background

CD107b, also known as LAMP2 (Lysosome-Associated Membrane Protein 2), is a heavily glycosylated type I transmembrane protein that constitutes a major component of the lysosomal membrane, where it plays critical roles in maintaining lysosomal integrity, autophagy, and chaperone-mediated autophagy by acting as a receptor for chaperone–substrate complexes on the lysosomal surface.

Picture

FC

Human PBMCs (human peripheral blood mononuclear cells) were fixed with 4% PFA and permeabilization with the 0.1% Tween. Cells were then intracellularly stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Alexa Fluor® 647 Mouse IgG1, k Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD107b/LAMP2 Antibody (right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0B88804), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.