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ZNF296 Recombinant Rabbit mAb (S-2675-58)

ZNF296 Recombinant Rabbit mAb (S-2675-58)

Catalog Number: S0B6492 Application: WB Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen ZNF296
Synonyms Zinc finger protein 296; ZFP296; Zinc finger protein 342; ZNF342
Immunogen Synthetic Peptide
Location Nucleus
Accession Q8WUU4
Clone Number S-2675-58
Antibody Type Recombinant mAb
Isotype IgG
Application WB
Reactivity Hu
Positive Sample Raji, Ramos
Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu

Background

ZNF296 is a C2H2-type zinc-finger transcription factor of 347–445 amino acids that localizes to the nucleus, binds specific double-stranded DNA sequences, and serves pleiotropic roles in embryonic stem-cell pluripotency, spermatogenesis, and cardiovascular morphogenesis by orchestrating chromatin accessibility and alternative splicing of genes such as mef2ca, sparc and tnnt3b, while its dysregulation has been linked to breast cancer, latent tuberculosis, doxorubicin-induced cardiotoxicity and acute myeloid leukaemia, making it an emerging disease biomarker.

Picture

Western Blot

WB result of ZNF296 Recombinant Rabbit mAb
Primary antibody: ZNF296 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-1080 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Negative control: HT-1080 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 55-65 kDa