WB result of YWHAH/14-3-3 eta Recombinant Rabbit mAb
Primary antibody: YWHAH/14-3-3 eta Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
YWHAH/14-3-3 eta Recombinant Rabbit mAb (S-1327-22)
YWHAH/14-3-3 eta Recombinant Rabbit mAb (S-1327-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | YWHAH/14-3-3 eta |
| Synonyms | Protein AS1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q04917 |
| Clone Number | S-1327-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Jurkat, HeLa, A431, NIH/3T3, PC-12 |
| Predicted Reactivity | Pr, Bv, Cz, Pg, Hm, Av, Hm |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
The 14-3-3 η protein is a member of the 14-3-3 protein family, which plays a significant role in various cellular processes, including signal transduction, cell cycle regulation, and apoptosis. This protein is found in high concentrations in certain tissues and has been identified as a potential biomarker for rheumatoid arthritis (RA). In the context of RA, 14-3-3 η protein has been found to be elevated in the blood and synovial fluid of patients with RA compared to healthy individuals. It is considered a promising diagnostic biomarker that can complement existing serological measurements like rheumatoid factor (RF) and anticyclic citrullinated peptide antibody (ACPA) to enhance the detection of RA. Furthermore, 14-3-3 η protein levels have been correlated with disease activity and joint damage in RA. It has been suggested that the protein may be involved in the pathogenesis of RA by stimulating the expression of factors that contribute to joint damage, such as matrix metalloproteinases (MMPs) and receptor activator of nuclear factor kappa-B ligand (RANKL).
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Western Blot
WB result of YWHAH/14-3-3 eta Recombinant Rabbit mAb
Primary antibody: YWHAH/14-3-3 eta Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
WB result of YWHAH/14-3-3 eta Recombinant Rabbit mAb
Primary antibody: YWHAH/14-3-3 eta Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- YWHAH/14-3-3 eta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- YWHAH/14-3-3 eta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
