WB result of YTHDF1 Recombinant Rabbit mAb
Primary antibody: YTHDF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
This blot was developed with high sensitivity substrate
YTHDF1 Recombinant Rabbit mAb (S-3433-13)
YTHDF1 Recombinant Rabbit mAb (S-3433-13)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | YTHDF1 |
| Synonyms | YTH domain-containing family protein 1; C20orf21 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm |
| Accession | Q9BYJ9 |
| Clone Number | S-3433-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HepG2, NIH/3T3, mouse testis, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:500 | Hu, Ms, Rt |
| IF | 1:50 | Hu, Ms |
Background
YTHDF1 (YTH N6-Methyladenosine RNA Binding Protein 1) is a cytoplasmic reader protein that specifically recognizes and binds to N6-methyladenosine (m6A), the most prevalent internal epitranscriptomic modification in eukaryotic messenger RNAs. Containing a conserved YTH (YT521-B homology) domain that mediates selective m6A recognition, YTHDF1 promotes translation efficiency of its target transcripts by facilitating ribosome loading and interacting with translation initiation factors, including eIF3 complex components. Through this mechanism, YTHDF1 enhances the expression of critical oncogenes and cell cycle regulators, thereby influencing cell proliferation, stem cell maintenance, and immune responses. Notably, YTHDF1 has emerged as a significant player in cancer biology, where its overexpression in various malignancies—including hepatocellular carcinoma, gastric cancer, and acute myeloid leukemia—correlates with tumor progression, metastasis, and poor clinical outcomes. Additionally, YTHDF1-mediated m6A recognition regulates antitumor immunity by modulating the translation of immune checkpoint molecules and cytokine transcripts in the tumor microenvironment. Beyond cancer, YTHDF1 contributes to synaptic plasticity and learning memory in the nervous system, as well as to viral replication through selective enhancement of viral mRNA translation. The therapeutic potential of targeting YTHDF1-m6A interactions is currently being explored through the development of small molecule inhibitors that disrupt this oncogenic axis.
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Western Blot
WB result of YTHDF1 Recombinant Rabbit mAb
Primary antibody: YTHDF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
This blot was developed with high sensitivity substrate
WB result of YTHDF1 Recombinant Rabbit mAb
Primary antibody: YTHDF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
This blot was developed with high sensitivity substrate
WB result of YTHDF1 Recombinant Rabbit mAb
Primary antibody: YTHDF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-YTHDF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-YTHDF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-YTHDF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-YTHDF1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-YTHDF1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
