| Species | Human |
| Accession | Q14191 |
| Amino Acid Sequence | N517-P1238，N-terminal 6xHis and zz tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 97.1 kDa |
| Purity | > 85% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
WRN titration and inhibitor IC50 determination
The WRN activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the WRN protein, ATP and dsDNA at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
M MW Standard
1 1 μg WRN
2 3 μg WRN
