Measured by its ability to induce TCF reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is less than 90ng/ml.
Wnt Surrogate Protein, Human
Wnt Surrogate Protein, Human
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$200 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Wnt3a Surrogate; Wnt Surrogate-Fc Fusion Protein; Human Wnt3a Surrogate Protein; Wnt Surrogate Fc Chimera,Human |
| Amino Acid Sequence | Lys177-His266 |
| Expression System | HEK293 |
| Molecular Weight | 57.5kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 25mM Tris.pH8.2,500mM NaCl,5%海藻糖 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Obrecht-Sturm, Schömig, Mynarek et al Neuro Oncol (2024) |
Background
Wnt surrogate protein is an engineered Fc fusion protein, which activates similar Wnt signaling pathways as most commercially available recombinant Wnt3a proteins or Wnt-conditioned media, while requiring a fraction of the amount of protein or hassle. It is necessary for cell proliferation and differentiation and the self-renewal of stem cells and is widely used for supporting organoid culture, including intestinal, stomach, liver, lung organoids. Modulation of WNT signaling pathways has potential for treatment of degenerative diseases and tissue injuries. This engineered Wnt surrogate protein can be selective for individual FZD or a subfamily of FZDs in combination with LRP5 or LRP6, depending on the binder specificities, and may be used in any of a variety of therapeutic methods for the treatment of diseases.
Recombinant Human Wnt3a Surrogate Protein activates Wnt signaling pathways and is necessary for cell proliferation and differentiation and the self-renewal of stem cells. It is widely used for supporting organoid culture, including intestinal, stomach, liver, lung organoids.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
