Flow cytometric analysis ScFv based CAR containing a Whitlow/218 linker transfected 293T (Human embryonic kidney epithelial cell, Right panel) or 293T (Left panel) was stained with Whitlow/218 Linker Recombinant Rabbit mAb (Biotin Conjugate) at
5 μl/test
followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Whitlow/218 Linker Recombinant Rabbit mAb (Biotin conjugate) (S-712-10)
Whitlow/218 Linker Recombinant Rabbit mAb (Biotin conjugate) (S-712-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Whitlow/218 Linker |
| Immunogen | Synthetic Peptide |
| Clone Number | S-712-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Purification | Protein A |
| Concentration | 20μg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Species independent |
Background
Whitlow/218 Linker is a peptide linker used in the construction of single-chain variable fragments (scFv), designed to reduce aggregation and enhance proteolytic stability. It plays a crucial role in chimeric antigen receptor (CAR) technology by improving the recognition capability and stability of scFv against target antigens, thereby serving as a key component in immunotherapy and biomedical research.
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