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    VPS18 Recombinant Rabbit mAb (S-2517-23)

    VPS18 Recombinant Rabbit mAb (S-2517-23)

    Catalog Number: S0B6416 Application: WB, IHC-P Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter
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    Product Details

    Product Specification


    Host Rabbit
    Antigen VPS18
    Synonyms Vacuolar protein sorting-associated protein 18 homolog; hVPS18; KIAA1475
    Immunogen Synthetic Peptide
    Location Lysosome, Endosome
    Accession Q9P253
    Clone Number S-2517-23
    Antibody Type Recombinant mAb
    Isotype IgG
    Application WB, IHC-P
    Reactivity Hu, Ms, Rt
    Positive Sample HeLa, 293T, K562, A549, HepG2, mouse brain, rat brain
    Purification Protein A
    Concentration 0.5 mg/ml
    Conjugation Unconjugated
    Physical Appearance Liquid
    Storage Buffer

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
    Stability & Storage

    12 months from date of receipt / reconstitution, -20 °C as supplied

    Dilution


    application dilution species
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:250 Hu, Ms, Rt

    Background

    VPS18 (Vacuolar Protein Sorting 18) is a conserved eukaryotic protein that serves as a core subunit of both the HOPS and CORVET tethering complexes, which mediate membrane docking and fusion events in the late endosomal and lysosomal pathways. It plays a critical role in vesicle trafficking, including endocytosis, autophagy, and lysosome biogenesis. VPS18 contains a RING-finger domain, suggesting E3 ubiquitin ligase activity, and has been implicated in viral replication, neurodegeneration, and tumor suppression through its regulation of receptor degradation and intracellular signaling pathways.

    Picture

    Western Blot

    WB result of VPS18 Recombinant Rabbit mAb
    Primary antibody: VPS18 Recombinant Rabbit mAb at 1/1000 dilution
    Lane 1: HeLa whole cell lysate 20 µg
    Lane 2: 293T whole cell lysate 20 µg
    Lane 3: K562 whole cell lysate 20 µg
    Lane 4: A549 whole cell lysate 20 µg
    Lane 5: HepG2 whole cell lysate 20 µg
    Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 110 kDa
    Observed MW: 110 kDa
    This blot was developed with high sensitivity substrate

    WB result of VPS18 Recombinant Rabbit mAb
    Primary antibody: VPS18 Recombinant Rabbit mAb at 1/1000 dilution
    Lane 1: mouse brain lysate 20 µg
    Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 110 kDa
    Observed MW: 110 kDa
    This blot was developed with high sensitivity substrate

    WB result of VPS18 Recombinant Rabbit mAb
    Primary antibody: VPS18 Recombinant Rabbit mAb at 1/1000 dilution
    Lane 1: rat brain lysate 20 µg
    Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 110 kDa
    Observed MW: 110 kDa
    This blot was developed with high sensitivity substrate

    Immunohistochemistry

    IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-VPS18 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.