ICC shows positive staining in HeLa cells. Anti- Vinculin (FITC Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Vinculin Recombinant Rabbit mAb (FITC Conjugate) (S-1635-6)
Vinculin Recombinant Rabbit mAb (FITC Conjugate) (S-1635-6)
Price:
Regular price
$180 USD
Regular price
Sale price
$180 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Vinculin |
| Synonyms | Metavinculin (MV); VCL |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cell junction, Cytoskeleton |
| Accession | P18206 |
| Clone Number | S-1635-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, NIH/3T3, C6 |
| Predicted Reactivity | Bv, Av, Xe |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:50 | Hu, Ms, Rt |
Background
Vinculin is a critical cytoskeletal protein that plays a significant role in cell adhesion, migration, and mechanical force transmission. It is highly enriched in regions where cells contact one another and the underlying substratum. Vinculin is involved in the formation and stabilization of focal adhesions, which are essential for cell-matrix interactions. It binds to several proteins, including talin, α-actinin, and paxillin, to link the actin cytoskeleton to integrins, facilitating cell adhesion and migration. Vinculin helps in transmitting mechanical forces from the extracellular matrix to the actin cytoskeleton, playing a crucial role in mechanotransduction. It can undergo conformational changes upon binding to different partners, which is essential for its function in force transmission. Vinculin stimulates actin polymerization and recruits actin remodeling proteins, which is vital for cell protrusion and migration.
Picture
Picture
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti- Vinculin (FITC Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti- Vinculin (FITC Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
