WB result of Versican Recombinant Rabbit mAb
Primary antibody: Versican Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MDA-MB-231 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: mouse hippocampus lysate 20 µg
Negative control: MDA-MB-231 whole cell lysate; HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 373 kDa
Observed MW: 300-400 kDa
This blot was developed with high sensitivity substrate
Versican Recombinant Rabbit mAb (S-3770-43)
Versican Recombinant Rabbit mAb (S-3770-43)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Versican |
| Synonyms | Versican core protein; Chondroitin sulfate proteoglycan core protein 2 (Chondroitin sulfate proteoglycan 2); Glial hyaluronate-binding protein (GHAP); Large fibroblast proteoglycan; PG-M; CSPG2; VCAN |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P13611 |
| Clone Number | S-3770-43 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse hippocampus, rat hippocampus |
| Predicted Reactivity | PiMq |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
Versican is a large extracellular matrix proteoglycan primarily secreted by mesenchymal cells and belonging to the hyaluronan-binding lectican family; its core structure comprises an N-terminal G1 domain (responsible for stable binding to hyaluronan and link proteins), a central region containing glycosaminoglycan (GAG) attachment domains (GAG-β and/or GAG-α) that are covalently linked to chondroitin sulfate chains to confer significant hydration capacity, turgor pressure, and elastic buffering to tissues, and a C-terminal G3 domain that mediates interactions with other matrix components such as fibulins, selectins, and growth factors. This protein is critical during embryonic development for heart formation, neural crest cell migration, and limb morphogenesis, while in adult tissues it plays a widespread role in vascular homeostasis, wound healing, and the regulation of inflammatory responses; moreover, its aberrant overexpression is closely associated with the remodeling of the tumor microenvironment, where it promotes cancer cell proliferation, migration, invasion, and immune evasion, establishing Versican as a key biomarker for the progression and poor prognosis of various malignant tumors.
Picture
Picture
Western Blot
WB result of Versican Recombinant Rabbit mAb
Primary antibody: Versican Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat hippocampus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 373 kDa
Observed MW: 300-400 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
