WB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: bEnd.3 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 88, 100-140 kDa
VE-Cadherin Recombinant Rabbit mAb (S-3312-5)
VE-Cadherin Recombinant Rabbit mAb (S-3312-5)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VE-Cadherin |
| Synonyms | Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CD144; CDH5 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cell junction |
| Accession | P33151 |
| Clone Number | S-3312-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Positive Sample | bEnd.3, mouse lung, rat lung, rat heart |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
Background
VE-Cadherin (vascular endothelial cadherin, CDH5) is a 140-kDa transmembrane glycoprotein that forms the core of endothelial adherens junctions, where its calcium-dependent homophilic interactions between extracellular EC1-EC5 repeats create a semi-permeable barrier between blood and tissues; its cytoplasmic tail recruits β-catenin, plakoglobin and p120-catenin, linking the junction to the actin cytoskeleton and enabling transmission of shear-stress signals that regulate angiogenesis, vascular remodeling and leukocyte extravasation, while phosphorylation of Y685 and Y658 by Src, VEGFR2 or Tie-2 and subsequent internalization of the complex transiently increases permeability during inflammation, tumor metastasis and atherosclerosis, making VE-cadherin a key therapeutic target in vascular pathologies.
Picture
Picture
Western Blot
WB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 100-120 kDa
WB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 100-140 kDa
