WB result of VDAC3 Rabbit pAb
Primary antibody: VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 30 kDa
VDAC3 Rabbit Polyclonal Antibody
VDAC3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VDAC3 |
| Synonyms | Non-selective voltage-gated ion channel VDAC3; VDAC-3; hVDAC3Alternative nameOuter mitochondrial membrane protein porin 3 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q9Y277 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK293, Jurkat, A431, MCF7, HeLa, HepG2, MEF, NIH/3T3, mouse brain, mouse heart, PC-12, rat brain, rat heart |
| Predicted Reactivity | BV, Pg, Rb, Or |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
VDAC3 (Voltage-Dependent Anion Channel 3) is a crucial isoform of the mitochondrial outer membrane porin family that distinguishes itself from its counterparts, VDAC1 and VDAC2, through unique structural features including an additional cysteine residue which facilitates the formation of intermolecular disulfide bonds and potential oligomerization. Primarily localized in the mitochondria of diverse tissues with particularly high expression in the brain, testes, and skeletal muscle, VDAC3 plays a specialized role in regulating metabolite flux, such as ATP and ADP exchange, while critically contributing to the assembly and stability of the sperm flagellar axoneme, where its dysfunction is directly linked to male infertility due to impaired sperm motility. Beyond its metabolic gating function, VDAC3 is implicated in modulating reactive oxygen species (ROS) levels and interacting with cytoskeletal elements, suggesting a broader involvement in cellular signaling pathways related to oxidative stress response and apoptosis, although its precise gating mechanisms and specific protein-protein interaction networks remain less fully characterized compared to the more ubiquitous VDAC1.
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Western Blot
WB result of VDAC3 Rabbit pAb
Primary antibody: VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 30 kDa
WB result of VDAC3 Rabbit pAb
Primary antibody: VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 30 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
