The VAV1 activity was assayed by HTRF technology. The reaction was performed by incubating the VAV1 protein and CRBN( UA080011 ), adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Accession | P15498-1 |
Amino Acid Sequence | K782-C845(end), with N-terminal GST tag |
Expression System | E.coli |
Molecular Weight | 33.9 kDa |
Purity | >90% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
