WB result of V5 Tag Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: V5 Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T transfected with Histone H3-Flag-V5 tag whole cell Lysate 20 µg
Predicted MW: 15 kDa
Observed MW: 17 kDa
V5 Tag Recombinant Rabbit mAb (HRP Conjugate) (S-609-8)
V5 Tag Recombinant Rabbit mAb (HRP Conjugate) (S-609-8)
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$105.00 SGD
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$105.00 SGD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Clone Number | S-609-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | null |
Background
V5-tag is a short peptide tag for detection and purification of proteins. The V5-tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and nanobodies.
Picture
Picture
Western Blot
