ICC shows positive staining in Histone H3-V5 tag transfected 293T cells. Anti-V5 Tag (FITC Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
V5 Tag Mouse mAb (FITC Conjugate) (S-610-66)
V5 Tag Mouse mAb (FITC Conjugate) (S-610-66)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Synthetic Peptide |
| Clone Number | S-610-66 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICC |
| Reactivity | Species Independent |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 |
Background
V5-tag is a short peptide tag for detection and purification of proteins. The V5-tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies.
Picture
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Immunocytochemistry
Negative control: ICC shows negative staining in vector-transfected 293T cells. Anti-V5 Tag (FITC Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
