IHC shows positive staining in paraffin-embedded human pituitary. Anti-TSHB antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
TSHB Recombinant Rabbit mAb (S-537-52)
TSHB Recombinant Rabbit mAb (S-537-52)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TSHB |
| Synonyms | Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta (TSH-B; TSH-beta), Thyrotropin beta chain |
| Immunogen | Synthetic Peptide |
| Accession | P01222 |
| Clone Number | S-537-52 |
| Antibody Type | Recombinant mAb |
| Application | IHC-P, IF |
| Reactivity | Hu, Rt |
| Predicted Reactivity | Ct, Dg, Hr, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:2000 | |
| IF | 1:200 |
Background
Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit (TSHA) and a unique beta subunit (this protein) that confers specificity.
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Picture
Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-TSHB antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pituitary. Anti-TSHB antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human pituitary. Anti-TSHB antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
