WB result of TRPV4 Recombinant Rabbit mAb
Primary antibody: TRPV4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled MCF7 whole cell lysate 20 µg
Lane 2: unboiled A375 whole cell lysate 20 µg
Negative control: unboiled MCF7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 98 kDa
Observed MW: 100 kDa
TRPV4 Recombinant Rabbit mAb (S-1746-65)
TRPV4 Recombinant Rabbit mAb (S-1746-65)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TRPV4 |
| Synonyms | Transient receptor potential cation channel subfamily V member 4; Osm-9-like TRP channel 4 (OTRPC4); Transient receptor potential protein 12 (TRP12); Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2 (VRL-2); Vanilloid receptor-related osmotically-activated channel (VR-OAC); VRL2; VROAC |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9HBA0 |
| Clone Number | S-1746-65 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A375, mouse kidney |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:200-1:500 | Hu, Ms, Rt |
Background
TRPV4 (Transient Receptor Potential Vanilloid 4) is a non-selective cation channel that belongs to the transient receptor potential (TRP) family. TRPV4 is a polymodal receptor that is activated by various stimuli, including warm temperature, osmotic changes, and mechanical stress. It is critically involved in physiological processes such as temperature sensation, osmoregulation, and mechanotransduction. TRPV4 is a calcium-permeable cation channel that is detectable in both sensory and nonsensory cells. It is characterized by multimodal activation properties, implicating it in a broad range of functions from osmoregulation to thermosensing. Its mutations are associated with several genetic diseases, such as bone dysplasia, early-onset osteoarthritis, and neuromotor dysfunction. TRPV4 is implicated in the pathophysiology of various cardiovascular diseases, including hypertension, atherosclerosis, myocardial ischemia-reperfusion injury, heart failure, pulmonary edema, and arrhythmias. Overactivation of TRPV4 can induce apoptosis, suggesting a role in cell death, which is relevant to cancer progression. Additionally, the expression level of TRPV4 in tumor cells is positively correlated with their metastatic ability, making TRPV4 a potential therapeutic target for inhibiting tumor metastasis.
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Western Blot
WB result of TRPV4 Recombinant Rabbit mAb
Primary antibody: TRPV4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse pancreas lysate 20 µg
Lane 2: unboiled mouse kidney lysate 20 µg
Negative control: unboiled mouse pancreas lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 98 kDa
Observed MW: 110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TRPV4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TRPV4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-TRPV4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded choroid plexus of rat brain. Anti-TRPV4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TRPV4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-TRPV4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
