WB result of TNFRSF17/BCMA Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: TNFRSF17/BCMA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U266B1 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 15-30 kDa
TNFRSF17/BCMA Recombinant Rabbit mAb (S-4674)
TNFRSF17/BCMA Recombinant Rabbit mAb (S-4674)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TNFRSF17/BCMA |
| Synonyms | Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD269 |
| Location | Cell membrane |
| Accession | Q02223 |
| Clone Number | S-4674 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | U266B1 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:100 | Hu |
| ICC | 1:500 | Hu |
Background
B-cell maturation antigen (BCMA, gene name TNFRSF17) is a member of the tumor necrosis factor receptor superfamily (TNFRSF) that is specifically expressed on late-stage B-cell lineage cells, including plasmablasts and long-lived plasma cells, while being virtually absent in normal tissues or early B-cells. As a key receptor for both BAFF (B-cell activating factor) and APRIL (a proliferation-inducing ligand), BCMA recruits TRAF adapter proteins to activate downstream signaling pathways such as NF-κB, MAPK, and PI3K/Akt, playing a central role in sustaining plasma cell survival, differentiation, and antibody secretion. Due to its highly restricted expression profile and its aberrant overexpression on multiple myeloma (MM) cells, BCMA has emerged as a premier therapeutic target; consequently, various BCMA-directed therapies, including chimeric antigen receptor T-cell (CAR-T) therapy, bispecific antibodies, and antibody-drug conjugates (ADCs), have demonstrated remarkable clinical efficacy, fundamentally transforming the treatment landscape for this disease.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-TNFRSF17/BCMA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TNFRSF17/BCMA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TNFRSF17/BCMA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TNFRSF17/BCMA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-TNFRSF17/BCMA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TNFRSF17/BCMA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in U266B1 cells (top panel) and negative staining in HeLa cells (below panel). Anti- TNFRSF17/BCMA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
