WB result of TIMP2 Recombinant Rabbit mAb
Primary antibody: TIMP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-1080 whole cell lysate 20 µg
Lane 2: A172 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: SW480 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 25 kDa
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TIMP2 |
| Synonyms | Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2 (TIMP-2) |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P16035 |
| Clone Number | S-3369-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Mk |
| Positive Sample | HT-1080, A172, U-87 MG, SW480, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
Picture
Picture
Western Blot
WB result of TIMP2 Recombinant Rabbit mAb
Primary antibody: TIMP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 25 kDa
