WB result of TFF1 Rabbit mAb Primary antibody: TFF1 Rabbit mAb at 1/1000 dilution Lane 1: MDA-MB-231 whole cell lysate 20 µg Lane 2: MCF7 whole cell lysate 20 µg Negative control: MDA-MB-231 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 13 kDa Observed MW: 13 kDa
TFF1 Recombinant Rabbit mAb (S-336-75)
TFF1 Recombinant Rabbit mAb (S-336-75)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TFF1 |
| Synonyms | Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A (hP1.A), Protein pS2, BCEI, PS2 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P04155 |
| Clone Number | S-336-75 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC | 1:500 | null |
Background
Trefoil factor 1 is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-TFF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-TFF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-TFF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TFF1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
