WB result of Tau (phospho S214) Recombinant Rabbit mAb
Primary antibody: Tau (phospho S214) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: old mouse brain lysate 20 µg
Lane 2: phosphatase treated old mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 45-55 kDa
Tau (phospho S214) Recombinant Rabbit mAb (S-1032-326)
Tau (phospho S214) Recombinant Rabbit mAb (S-1032-326)
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$130.00 SGD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Tau (phospho S214) |
| Synonyms | Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); MAPTL; MTBT1; TAU; MAPT |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Secreted, Cell membrane, Endoplasmic reticulum |
| Accession | P10636-8 |
| Clone Number | S-1032-326 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Positive Sample | old mouse brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Ms |
Background
Tau (phospho S214) protein is a phosphorylated form of the tau protein, which is a microtubule-associated protein primarily found in neurons of the central nervous system. It is encoded by the MAPT gene and plays a crucial role in maintaining the stability and promoting the assembly of microtubules in axons. The phosphorylation of tau at serine 214 (S214) by cAMP-dependent protein kinase A (PKA) has been shown to reduce the pathological assembly of the protein. This phosphorylation event is significant as it can influence the protein's binding affinity to microtubules and its involvement in neurofibrillary tangles, which are a hallmark of Alzheimer's disease and other tauopathies.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Tau (phospho S214) Recombinant Rabbit mAb
Lane 1: Tau (S214) phospho peptide
Lane 2: Tau unmodified peptide (around S214)
Primary antibody: Tau (phospho S214) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
