WB result of STAT5 Rabbit mAb
Primary antibody: STAT5 Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
Exposure time: 180 s (This blot was developed with high sensitivity substrate)
STAT5 Recombinant Rabbit mAb (S-R234)
STAT5 Recombinant Rabbit mAb (S-R234)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | STAT5 |
| Synonyms | Signal transducer and activator of transcription 5A; STAT5A |
| Location | Cytoplasm, Nucleus |
| Accession | P42229 |
| Clone Number | S-R234 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC | 1:100-1:200 |
Background
Signal transducer and activator of transcription 5 (STAT5) refers to two highly related proteins, STAT5A and STAT5B, which are part of the seven-membered STAT family of proteins. STAT5 proteins are involved in cytosolic signalling and in mediating the expression of specific genes. Aberrant STAT5 activity has been shown to be closely connected to a wide range of human cancers, and silencing this aberrant activity is an area of active research in medicinal chemistry. STAT5 has been found to be constitutively phosphorylated in cancer cells, implying that the protein is always present in its active form. This constant activation is brought about either by mutations or by aberrant expressions of cell signalling, resulting in poor regulation, or complete lack of control, of the activation of transcription for genes influenced by STAT5. This leads to constant and increased expression of these genes.
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Picture
Western Blot
IP
STAT5 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating STAT5 in 0.4 mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using STAT5 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: K562 whole cell lysate 40 µg (Input)
Lane 2: STAT5 Recombinant Rabbit mAb IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 90 kDa
Observed MW: 90 kDa
Exposure time: 30 s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-STAT5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-STAT5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-STAT5 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-STAT5 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
