WB result of ALOX15 Rabbit mAb
Primary antibody: ALOX15 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 70 kDa
(This blot was developed with high sensitivity substrate)
ALOX15 Recombinant Rabbit mAb (S-669-56)
ALOX15 Recombinant Rabbit mAb (S-669-56)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Polyunsaturated fatty acid lipoxygenase ALOX15, 12/15-lipoxygenase, leukocyte-type Arachidonate 12-lipoxygenase (12-LOX), Arachidonate 15-lipoxygenase (15-LOX; 15-LOX-1), Arachidonate omega-6 lipoxygenase, Hepoxilin A3 synthase Alox15, Linoleate 13S-lipoxygenase |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P16050 |
| Clone Number | S-669-56 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Predicted Reactivity | Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:1000 |
Background
The ALOX15 protein is encoded by the Alox15 gene and functions as a non-heme iron-containing dioxygenase that catalyzes and esterifies the peroxidation of polyunsaturated fatty acids, producing a series of bioactive lipid intermediates. This enzyme inserts a peroxy group at the C12 or C15 position of arachidonic acid esters, generating 12-HPETE and 15-HPETE, and further metabolizes 12-HPETE to produce pro-inflammatory factors such as hepoxilins. Additionally, Alox15 can also peroxide linoleic acid esters to 13-HPODE and participate in the oxidation of DHA to produce specific pro-resolving mediators (SPMs), such as resolvin D5 and (7S,14S)-diHPDHA, which actively down-regulate immune responses and have anti-platelet aggregation properties. Beyond its biochemical functions, the ALOX15 protein also has important applications in the medical field. Studies have shown that recombinant ALOX15 protein can increase the level of interferon IFNβ in the body, effectively blocking pathogenic infections, independent of its enzymatic activity. Molecular mechanism studies have confirmed that the ALOX15 protein promotes the K63 deubiquitination modification of the mitochondrial protein MAVS, increasing the aggregation and activation of MAVS, and subsequently inducing the secretion of IFNβ. Therefore, the recombinant ALOX15 protein can be applied to enhance immunity and prevent and treat pathogenic infections and their complications, including influenza A and B viruses, novel coronavirus, Mycobacterium tuberculosis, Chlamydia trachomatis, and others.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-ALOX15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
