Flow cytometric analysis of CD324 expression on HT-29 cells. Cells from the HT-29 (Human colorectal adenocarcinoma epithelial cell, Right) or MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) was stained with either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Rat Anti-Mouse/Human CD324 Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse/Human CD324 Antibody (S-R650)
Alexa Fluor® 647 Rat Anti-Mouse/Human CD324 Antibody (S-R650)
Price:
Regular price
$95 USD
Regular price
Sale price
$95 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD324 |
| Synonyms | Cadherin-1; CAM 120/80; Epithelial cadherin (E-cadherin); Uvomorulin; CDHE; UVO; CDH1 |
| Location | Cytoplasm, Endosome, Cell membrane |
| Accession | P12830 |
| Clone Number | S-R650 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | HT-29 |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu, Ms |
Background
CD324, also known as E-cadherin or cadherin-1, is a calcium-dependent cell adhesion protein that plays a crucial role in maintaining epithelial tissue integrity and regulating cell proliferation, differentiation, and survival. It is a member of the cadherin superfamily and is widely expressed on the cell surface of most epithelial tissues. The extracellular domain of CD324 facilitates homophilic binding between adjacent cells, while its cytoplasmic domain interacts with the actin cytoskeleton through catenins. This protein is essential for epithelial cell cohesion and acts as an invasion suppressor, with its loss often indicating high tumor aggressiveness in cancer.
Picture
Picture
FC
