Flow cytometric analysis of Mouse CD21/CD35 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse CD21/CD35 antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse CD21/CD35 antibody (S-R717)
Alexa Fluor® 647 Rat Anti-Mouse CD21/CD35 antibody (S-R717)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD21/CD35 |
| Synonyms | Complement receptor type 2; Complement C3d receptor; Cr2; Cr1 |
| Location | Cell membrane |
| Accession | P19070 |
| Clone Number | S-R717 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD21 (CR2) and CD35 (CR1) are key complement receptors predominantly expressed on B cells and follicular dendritic cells (FDCs). CD21, also known as the C3d receptor, binds to complement fragments like C3d and iC3b, playing a crucial role in B cell activation and regulation of immune responses. It also serves as the receptor for Epstein-Barr virus (EBV) on B cells. CD35, on the other hand, binds to C3b and C4b, facilitating phagocytosis and immune complex clearance. It helps in the activation of B cells and regulation of complement activity. Both receptors are involved in antigen presentation and immune complex internalization, contributing to both innate and adaptive immune responses.
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