Flow cytometric analysis of Human CD123 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 605™ Mouse Anti-Human HLA/DR antibody and either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD123 Antibody (Right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Human CD123 Antibody (S-R442)
Alexa Fluor® 488 Mouse Anti-Human CD123 Antibody (S-R442)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD123 |
| Synonyms | Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL3RA; IL3R |
| Location | Cell membrane |
| Accession | P26951 |
| Clone Number | S-R442 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.2mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25 μl per million cells in 100μl volume | Hu |
Background
The CD123 protein, also known as the Interleukin-3 receptor subunit alpha (IL-3RA) or CD123, is a crucial component of the interleukin-3 (IL-3) receptor complex and belongs to the Type I cytokine receptor family, specifically the Class 5 subfamily. CD123 serves as the high-affinity receptor for IL-3, forming a heterodimeric receptor complex with the β chain (CSF2RB). This protein mediates the stimulatory effects of IL-3 on the proliferation and differentiation of various hematopoietic cells, including pluripotent hematopoietic stem cells and lineage-committed cells. CD123 is expressed on normal plasmacytoid dendritic cells, basophils, monocytes, and eosinophils, and is overexpressed in various hematologic malignancies. In clinical research, the expression of CD123 is associated with chemotherapy resistance and high-risk genetic alterations in certain leukemias, such as acute myeloid leukemia (AML).
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