1μg (R: reducing condition, N: non-reducing condition).
ALCAM/CD166 His Tag Protein, Human
ALCAM/CD166 His Tag Protein, Human
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$120 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD166 antigen，Activated leukocyte cell adhesion molecule，CD166，ALCAM，MEMD，Activated Leucocyte Cell Adhesion Molecule，Activated-Leukocyte Cell Adhesion Molecule |
| Accession | Q13740-1 |
| Amino Acid Sequence | Trp28-Ala526 with His Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 70-90kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE &＞90% by SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21. |
Background
Activated Leukocyte Cell Adhesion Molecule (ALCAM), also known as Cluster of Differentiation 166 (CD166), is a type I transmembrane cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily. It serves as a ligand for CD6 and is expressed on T lymphocytes. The interaction between ALCAM and CD6 is crucial for T cell development, regulation, and for the binding of T- and B-cells to activated leukocytes.
ALCAM is expressed in a variety of tissues but is typically restricted to cell subsets involved in dynamic growth and/or migration. This includes processes such as neural development, branching organ development, hematopoiesis, immune response, and tumor progression. High expression levels of ALCAM on melanoma cell lines seem to promote metastasis, whereas in breast cancer, it has been associated with anti-metastatic activity.
ALCAM may also influence the expression or adhesion of the neuronal adhesion molecule NCAM-L1, as observed in both the developing retina and invasive melanoma.
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SDS-PAGE
SEC-HPLC
The purity of ALCAM/CD166 His Tag Protein, Human is greater than 90% as determined by SEC-HPLC.
ELISA
Immobilized ALCAM/CD166 His Tag Protein, Human (Cat. No. UA011112) at 5 μg/mL (100μL/well) can bind Human CD6, hFc tag (Cat. No. S0A1088) with EC50 of 0.14-0.18 μg/mL.
