WB result of ADAM9 Recombinant Rabbit mAb
Primary antibody: ADAM9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: U-87 MG whole cell lysate 20 µg
Lane 6: WI-38 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 75, 100 kDa
ADAM9 Recombinant Rabbit mAb (S-3270-74)
ADAM9 Recombinant Rabbit mAb (S-3270-74)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ADAM9 |
| Synonyms | Disintegrin and metalloproteinase domain-containing protein 9; ADAM 9; Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase; KIAA0021; MCMP; MDC9; MLTNG |
| Immunogen | Recombinant Protein |
| Location | Secreted, Cell membrane |
| Accession | Q13443 |
| Clone Number | S-3270-74 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Mk |
| Positive Sample | HeLa, HepG2, A549, A431, U-87 MG, WI-38, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:200 | Hu |
Background
ADAM9 (A Disintegrin And Metalloproteinase 9) is a transmembrane glycoprotein belonging to the ADAM family of membrane-anchored metalloproteinases, characterized by a multi-domain structure comprising a prodomain, metalloprotease domain with zinc-binding catalytic site, disintegrin domain mediating cell-cell and cell-matrix interactions, cysteine-rich region, EGF-like domain, transmembrane helix, and cytoplasmic tail. As a sheddase, ADAM9 cleaves diverse substrates including epidermal growth factor receptor (EGFR) ligands (such as HB-EGF and amphiregulin), amyloid precursor protein (APP), Notch receptors, and adhesion molecules, thereby regulating signaling pathways involved in cell proliferation, migration, and adhesion. Beyond its proteolytic activity, ADAM9 functions as an adhesion molecule through its disintegrin domain, interacting with integrins (particularly α6β1 and α9β1) and extracellular matrix components like fibronectin and gelatin. Dysregulated ADAM9 expression correlates with pathological conditions, notably promoting tumor progression, metastasis, and angiogenesis in various cancers (including breast, prostate, and pancreatic cancer) by facilitating cancer cell invasion and shedding of membrane-bound growth factors, while also implicating roles in inflammatory responses and neurodegenerative diseases such as Alzheimer's disease.
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Western Blot
WB result of ADAM9 Recombinant Rabbit mAb
Primary antibody: ADAM9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 75, 100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-ADAM9 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous carcinoma. Anti-ADAM9 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ADAM9 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
