WB result of 53BP1 Recombinant Rabbit mAb
Primary antibody: 53BP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 214 kDa
Observed MW: 350 kDa
53BP1 Recombinant Rabbit mAb (S-3170-149)
53BP1 Recombinant Rabbit mAb (S-3170-149)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | 53BP1 |
| Synonyms | TP53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1; TP53BP1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q12888 |
| Clone Number | S-3170-149 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HT-29, HCT 116 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
53BP1 (p53-binding protein 1) is a large, multi-domain scaffold protein that rapidly accumulates in irradiation-induced foci at DNA double-strand breaks, where it acts as a master regulator of the non-homologous end-joining repair pathway by binding phosphorylated H2AX (γH2AX) and histone H4 methylated on Lys20, thereby recruiting the downstream effector kinase ATM, the ubiquitin ligases RNF8/RNF168, and the adaptor proteins BRCA1 and RIF1 to antagonize BRCA1-mediated DNA-end resection and channel repair toward the error-prone but fast NHEJ route, while its own recruitment and retention are governed by tandem Tudor and BRCT domains that sense combinatorial histone marks, and its loss or mutation leads to genomic instability, hypersensitivity to ionizing radiation, and impaired class-switch recombination in B lymphocytes.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
